Publications

All publications (38)

Pavel Vopalensky, Ondřej Nešuta, Maria-Bianca Mititelu, Anton Škríba, Ambra Spampinato, Klára Viktorinová, Zuzana Buchová, Jana Březinová, Paul E. Reyes-Gutierrez, Ondřej Lukšan and Hana Cahova

Detection and sequencing of Ap2N-capped RNAs in human cells
PNAS 123 (38), e2608168123, 10.1073/pnas.2608168123

Valentina M. Serianni, Jana Škerlová, Anna Knopp Dubánková, Anton Škríba, Hana Šváchová, Tereza Vučková, Anatolij Filimonenko, Milan Fabry, Pavlína Řezáčová, Tomáš Kouba & Hana Cahova

Molecular insight into 5′ RNA capping with NpnNs by bacterial RNA polymerase
Nature Chemical Biology 2026 https://doi.org/10.1038/s41589-025-02134-5

Stanislav Stejskal, Veronika Rájecká, Helena Covelo-Molares, Ketty Sinigaglia, Jana Melicherová, Shwetha Krishna, Pavla Linhartova, Linda Kasiarova, Paul Eduardo Reyes-Gutierrez, Hana Cahová, Angela Gallo, Ales Obrdlik, Liam P. Keegan, Mary A. O’Connell, and Stepanka Vanacova

Global analysis by LC-MS/MS of N6-methyladenosine and inosine in mRNA reveal complex incidence
RNA 2025 10.1261/rna.080324.124

Pavel Vopalensky, Anton Škríba, Michela Chiumenti,  Lucia Ďuričeková, Anna Šimonová, Ondřej Lukšan,  Francesco di Serio,  Beatriz Navarro,  Hana Cahova

Exploring RNA modifications in infectious non-coding circular RNAs
RNA biology 2025 https://doi.org/10.1080/15476286.2025.2459039

Flaminia Mancini & Hana Cahova

The Mysterious World of Non-canonical Caps – What We Know and Why We Need New Sequencing Techniques
ChemBioChem 2024 https://doi.org/10.1002/cbic.202400604

Jiří František Potužník & Hana Cahova

If the 5’ cap fits (wear it) – Non-canonical RNA capping
RNA biology 2024 21(1), 1–13. https://doi.org/10.1080/15476286.2024.2372138

Barbora Benoni, Jiří František Potužník, Anton Škríba, Roberto Benoni, Jana Trylcova, Matouš Tulpa, Kristína Spustová, Katarzyna Grab, Maria-Bianca Mititelu, Jan Pačes, Jan Weber, David Stanek, Joanna Kowalska, Lucie Bednarova, Zuzana Keckesova, Pavel Vopalensky, Lenka Gahurova, and Hana Cahova 

HIV-1 Infection Reduces NAD Capping of Host Cell snRNA and snoRNA
ACS Chemical Biology 2024 doi.org/10.1021/acschembio.4c00151

Pavel Vopalensky, Anton Škríba, Michela Chiumenti,  Lucia Ďuričeková, Anna Šimonová, Ondřej Lukšan,  Francesco di Serio,  Beatriz Navarro,  Hana Cahová

Exploring RNA modifications in infectious non-coding circular RNAs
bioRxiv https://doi.org/10.1101/2024.03.12.584625
Arabidopsis thaliana NudiXes have RNA-decapping activity
RSC Chemical Biology 4 2023, 223-228

Šimonová A., Romanská V., Benoni B., Škubník K., Šmerdová L., Procházková M., Spustová K., Moravčík O., Gahurova L., Pačes J., Plevka P. & Cahová H.

Honeybee iflaviruses pack specific tRNA fragments from host cells in their virions
ChemBioChem 23 2022, e202200281.

Benoni R., Krafčíková P., Baranowski M.R., Kowalska J., Bouřa E. & Cahova H.

Substrate Specificity of SARS-CoV-2 Nsp10-Nsp16 Methyltransferase.
Viruses 13 1722, 2021

Hudeček O. & Cahova H.

Alarmony, ochránci bakteriální RNA
Vesmir 99 74, 2020

Potužník J. F. & Cahova H.

It’s the Little Things (in Viral RNA)
mBio 11 e02131-20, 2020

Benoni R., Culka M., Hudecek O., Gahurova L.& Cahova H.

Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation
ACS Chemical Biology doi.org/10.1021/acschembio.0c00178

Hudeček, O.,* Benoni R.,* Reyes-Gutierrez P.E., Culka M., Šanderová H.,
Hubálek M., Rulíšek L., Cvačka J., Krásný L. & Cahová H.

Dinucleoside polyphosphates act as 5′-RNA caps in bacteria
Nature Communications 11 1052, 2020

Hudecek, O.; Benoni, R.; Culka, M.; Hubalek, M.; Rulisek, L.; Cvacka, J.; Cahova, H.:

Dinucleoside polyphosphates act as 5′-RNA caps in Escherichia coli
bioRxiv

Šimonová A., Svojanovská B., Trylčová J., Hubálek M., Moravčík O., Zavřel M., Pávová M., Hodek J., Weber J., Cvačka J.,

Pačes J., Cahová H.

LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
Scientific Reports 9: 8697, 2019

Slavíčková M., Janoušková M., Šimonová A., Cahová H., Kambová M., Šanderová H., Krásný L., Hocek M.:

Turning off transcription with bacterial RNA polymerase through CuAAC click reactions of DNA containing 5-ethynyluracil.
Chem. Eur. J. 24: 8311-8314, 2018.

Cahová, H.

Prokaryotes also Protect their RNAs – New Types of 5′ RNA Caps. (invited review, in Czech)
Chemické listy 111: 414-423, 2017

Winz M.-L., Cahová H., Nübel G., Frindert J., Höfer K., Jäschke A.

Capture and sequencing of NAD-capped RNA sequences with NAD captureSeq.
Nature Protocols 12 122-149, 2017.

Cahová H., Panattoni A., Kielkowski P., Fanfrlík J., Hocek M.:

5-Substituted Pyrimidine and 7-Substituted 7-Deazapurine dNTPs as Substrates for DNA Polymerases in Competitive Primer Extension in the Presence of Natural dNTPs.
ACS Chemical Biology 11: 3165-3171, 2016

Daďová J., Cahová H., Hocek M.:

Polymerase Synthesis of Base-Modified DNA.
K. Nakatani, Y. Tor (eds.), Modified Nucleic Acids, Nucleic Acids and Molecular Biology Series Vol. 31: 123-144, Springer 2016

Kielkowski P., Cahová H., Pohl R., Hocek M.:

Flexible double-headed cytosine-linked 2′-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases.
Bioorganic & Medicinal Chemistry 24: 1268-1276, 2016

Cahová, H.; Winz M.-L.; Höfer K., Nübel G.; Jäschke A.:

NAD captureSeq indicates NAD as a bacterial cap for a subset of regulatory RNAs
Nature 519 374-377, 2015

Cahová, H.; Jäschke A.:

Nucleoside-Based Diarylethene Photoswitches and Their Facile Incorporation into Photoswitchable DNA
Angew. Chem. Int. Ed. 52 2013, 3186-3190

Ménová, P.; Cahová, H.; Plucnara, M.; Havran, L.; Fojta, M.; Hocek, M.:

Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling
Chem. Commun. 49 2013, 4652-4654

Kielkowski, P.; Macíčková-Cahová, H.; Pohl, R.; Hocek, M.:

Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases
Angew. Chem. Int. Ed. 50 2011, 8727 –8730

Macíčková-Cahová, H.; Pohl, R.; Horáková, P.; Havran, L.; Špaček, J.; Fojta, M.; Hocek, M.:

Alkylsulfanylphenyl Derivatives of Cytosine and 7-Deazaadenine Nucleosides, Nucleotides and Nucleoside Triphosphates: Synthesis, Polymerase Incorporation to DNA and Electrochemical Study
Chem. Eur. J. 17 2011, 5833–5841

Horáková, P.; Macíčková-Cahová, H.; Pivoňková, H.; Špaček, J.; Havran, L.; Hocek, M.; Fojta, M.:

Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays
Org. Biomol. Chem. 9, 2011,1366 – 1371

Macíčková-Cahová, H.; Pohl, R.; Hocek, M.:

Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases
ChemBioChem 12 2011, 431 – 438

Macíčková-Cahová, H.; Vrábel, M.; Hocek, M.:

Cross-Coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA
Curr. Protoc. Chem Biol. 2 2010, 1-14

Ikonen, S.; Macíčková-Cahová, H.; Pohl, R.; Šanda, M.; Hocek, M.:

Synthesis of nucleoside and nucleotide conjugates of bile acids, and polymerase construction of bile acid-functionalized DNA
Org. Biomol. Chem. 8 2010, 1194-1201

Macíčková-Cahová, H.; Hocek, M.:

Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
Nucleic Acids Res. 37, 2009, 7612–7622

Vrábel, M.; Horáková, P.; Pivoňková, H.; Kalachova, L.; Černocká, H.; Cahová, H.; Pohl, R.; Šebest, P.; Havran, L.; Hocek, M.; Fojta, M.:

Base-Modified DNA Labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ Complexes: Construction by Polymerase Incorporation of Modified Nucleoside Triphosphates, Electrochemical and Luminescent Properties, and Applications
Chem. Eur. J. 15, 2009, 1144-1154

Cahová, H.; Pohl, R.; Bednárová, L.; Nováková, K.; Cvačka, J.; Hocek, M.:

Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA
Org. Biomol. Chem. 6 2008, 3657-3660

Cahová, H.; Havran, L.; Brázdilová, P.; Pivoňková, H.; Pohl, R.; Fojta, M.; Hocek, M.:

Aminophenyl- and Nitrophenyl-Labeled Nucleoside Triphosphates: Synthesis, Enzymatic Incorporation, and Electrochemical Detection
Angew. Chem. Int. Ed. 47 2008, 2059–2062

Čapek, P.; Cahová, H.; Pohl, R.; Hocek, M.; Gloeckner, C.; Marx, A.:

An Efficient Method for the Construction of Functionalized DNA Bearing Amino Acid Groups through Cross-Coupling Reactions of Nucleoside Triphosphates Followed by Primer Extension or PCR
Chem. Eur. J. 13 2007, 6196–6203